Our laboratory in the Department of Pathology at Brigham and Women's Hospital and Harvard Medical School in Boston, MA, studies the immunobiology of innate T cells. We are particularly interested in the mechanisms that activate CD1d-restricted Natural Killer T (NKT) cells and MR1-restricted Mucosal-Associated Invariant T (MAIT) cells, and their role and function during inflammation and microbial infection. Current projects in the lab are focused on understanding the recognition of MR1-presented microbial antigens, the mechanisms of activation of MAIT cells, critical interactions with antigen presenting cells (APC), and their role and function during inflammation and microbial infection. The postdoctoral position involves using cutting edge molecular biology, cellular immunology, and chemistry techniques, as well as novel genetically modified in vivo mouse models, and collaborating extensively with local, national and international research groups. For more information on the Brigl lab, please visit: http://brigl.bwh.harvard.edu/
Characterize the role of MAIT cells during intestinal infection and in intestinal microbial homeostasis, including critical interactions with antigen presenting cells (APC) that regulate the activation and function of MAIT cells during homeostasis, inflammation and infection. This includes in vitro and in vivo analysis with various antigen formulations and in vitro or in vivo derived APCs, use of flow cytometry of immune cells, protein and gene expression analysis, and ELISA of cytokines secreted from lymphocytes and tissues, as well as in vitro and in vivo infections. Furthermore, novel conventional and inducible knockout mouse strains have been generated and will be characterized and deployed.
Investigate the role of pharmacologic activation of MAIT cells on intestinal and general immune function, including the impact on protective immunity, colonization resistance, and the possible adjuvant activity of MAIT cells, using cutting edge molecular biology, cellular immunology, and chemistry techniques, including RNAseq, as well as novel genetically modified mouse models.
Critically analyze obtained data, prepare data for publication and presentation, and with supervision from PI, prepare manuscripts for submission.
Maintains established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control.
Performs all other duties and responsibilities as directed.
PhD, MD, and/or DVM with previous experience in cellular immunology, molecular biology, and immunity to infection.
After appropriate training must be able to perform standard cellular and molecular biology techniques as listed above. Must be able to perform experiments and analyze data independently. Must have ability to write scientific English clearly with supervision from PI.
Posting ID: 547779760Posted: 2021-01-21