RESEARCH SCIENTIST / ENGINEER 3

    University of Washington
    Seattle, WA 98101
    Full-time, Part-time
    Similar jobs pay $16.65 - $32.71

    Job Description

    As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

    UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a "Great College to Work For" for six consecutive years.

    The University of Washington, Department of Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.

    UW Pathology brings together state-of-the-art technology and highly trained, world renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Pathology faculty members also serve at Seattle Children's Hospital, the Veteran's Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic pathology.

    The Department of Pathology has an outstanding opportunity for a full-time Research Scientist/Engineer 3.

    The Heart Regeneration Program, a translational program within UW Medicine, is developing human embryonic stem cell-derived cardiomyocytes as a cell therapeutic. Under the general guidance of Senior Scientists, this position will require to work independently to reach established milestones and goals. This position will also be expected to work collaboratively with fellow research personnel, and redirect efforts to achieve task/project objectives or enhance the quality of the project when necessary.

    The typical job functions for this position will include:

    • Cell culture responsibilities:
    o Aseptic and sterile work techniques
    o Expansion and maintenance of undifferentiated cell lines
    o Banking of undifferentiated human pluripotent stem cells (hPSCs)
    o Performing quality control assays for undifferentiated cell banks (karyotyping, mycoplasma testing, etc.)
    o Clonal isolation, expansion and banking of edited cell lines
    o Differentiation of human pluripotent stem cells towards various cardiovascular lineages (i.e. cardiomyocytes, epicardial cells, endothelial cells, smooth muscle cells)
    o Large scale banking of cardiomyocytes derived from hPSCs
    o Transfection of cultured cells
    • Perform molecular procedures such as DNA cloning and editing, plasmid preparations, and the isolation of DNA, RNA, and protein from cells and tissues;
    • Perform molecular analyses such as RT-PCR, western blotting, immunocytochemistry, and flow cytometry;
    • Assist with laboratory operations including ordering, regulatory compliance, and other key support as needed;
    • Prepare and maintain accurate protocols;
    • Assist in general lab maintenance;
    • Manage projects of moderate size and scope;
    • Perform tasks/projects within a larger scope, but lead specific tasks within the project's goals;
    • Develop protocols for tasks relevant to hers/his project;
    • Take responsibility for assuring quality, cost effectiveness and timeliness for specific projects;
    • Create, monitor, and implement effective plans;
    • Take lead in project/tasks improvements;
    • Contribute data towards lab's grants, publications, and presentations;
    • Provide ideas to improve efficiency at group level;
    • Mentor trainees and junior staff in development of technical skills;
    • Attend meetings as required;
    • Other duties as assigned

    Requirements include:

    • Bachelor's degree in a life science or related field
    • At least 3 years of experience in cell culture and molecular skills
    • Expertise in multi-color intracellular flow cytometry, and PCR
    • Excellent organizational and communication skills
    • Excellent writing skills
    • Ability to teach
    • Command of Microsoft Word, Excel, PowerPoint programs
    • Ability to work independently and as part of a diverse team
    • Ability to problem solve within the scope of a given project

    Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

    Desired:

    • Hands-on experience with human ESC or iPSC culture and differentiation;
    • M.S. in cell and developmental biology, physiology, biochemistry, or biomedical engineering.

    As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.

    Application Process:

    The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

    Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.

    The University of Washington is a leader in environmental stewardship & sustainability, and committed to becoming climate neutral.

    The University of Washington is an equal opportunity, affirmative action employer. To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 / 206-543-6452 (tty) or dso@uw.edu.

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    Posting ID: 554941371Posted: 2020-06-25