Working under general supervision, the Research Assistant 2 will plan and carry out research project in accordance with general plans. Duties include maintaining lab organization and mouse colonies. The research lab performs cutting-edge biomedical research to discover new features of the biology of cells and to understand their contribution to human disease, especially cancer. The lab is using our discoveries to develop new approaches to cancer treatment. The position involves work with animals.
- Organize and maintain mouse colony, keep clear records of mouse lines, genotype and wean pups. (25%)
- Plan and carry out experiments including PCR, gel electrophoresis, cryosectioning, immunohistochemistry, confocal microscopy, Western blotting, and basic molecular biology and protein biochemistry. May assist in developing improved techniques, projection methods or procedures. Collect and analyze data and prepare graphs, charts, and slides to report experimental data. (20%)
- Perform laboratory maintenance and organization under general guidance. Responsible for tracking and maintaining supply inventory, work with fund manager, laboratory personnel, and supply company representatives to ensure supplies are ordered cost effectively and in a timely manner and on correct funding source, responsible for maintenance of laboratory equipment in good working order, appropriately calibrated and maintained, ensure a clean, orderly, safe working environment. (25%)
- Maintain laboratory stocks and reagents, laboratory safety protocols and compliance, records of equipment manuals and procedures, maintain records of purchases, purchases received and outstanding, packing slips, and storage destination, and maintain records of laboratory experimental procedures and data. (15%)
- Coordinate laboratory safety with the department of occupational and environmental safety. Ensure compliance with protocols for safe handling and storage of hazardous materials, orient new personnel and direct them to obtain appropriate training through the department of occupational and environmental safety, maintain records of personnel training to ensure compliance with mandatory training requirements, periodically inspect the laboratory to ensure safe operation compliant with all safety protocols, work with the department of occupational and environmental safety to ensure compliance with all rules and regulations regarding chemical, biohazard, radiochemical, and general laboratory hazards. (10%)
Perform other duties as assigned. (5%)
Department: Frequent contact with supervisor and lab members to discuss research and maintain workflow.
University: Occasional contact with Environmental Health and Safety to ensure safety compliance.
External: Regular contact with supply and equipment vendors to obtain quotes and to ensure cost effective purchasing and maintenance of laboratory equipment.
Postdoc and Students: Frequent contact with postdoc and student employees to provide orientation to the lab, explain policies and procedures, coordinate purchasing, and collaboration on experiments.
This position has no direct supervision of staff employees. Orient new staff; direct the work of students.
Education and Experience: Bachelor of Science degree and 1 to 3 years of related experience or Associate's degree in an approved biotechnology program and 2 to 4 years of experience.
- Knowledge of commonly-used concepts, practices, and procedures within a biomedical research laboratory environment, including sterile technique and basic cell culture.
- Relies on instructions and pre-established guidelines to perform the functions of the job.
- Ability to operate laboratory equipment.
- Ability to meet consistent attendance.
- Must demonstrate compliance with university animal research care policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
- Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center's mission. Must handle animals with care and respect at all times.
- Must be able and willing to learn new techniques, procedures, and processes to protect the health of the animals.
- Previous experience working with animals preferred.
- Strong molecular biology skills (PCR, Western blots, subcloning).
- Strong organization skills and good habit of maintaining a clean lab working environment; demonstrate attention to detail and accuracy, time management skills, and proven ability to successfully follow- through on assigned projects.
- Professional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals within and external to the University.
- Ability to work effectively, independently, and collaboratively within a team (must be highly motivated, responsible, dependable and a self-starter).
- Proficiency in Microsoft Office and comfort with Mac and Windows computers.
- Must have the ability to maintain meticulous, complete, and easily retrievable laboratory data.
- Must have the ability to willingly learn new techniques and procedures as needed, follow established protocols or laboratory procedures and request clarification if necessary.
- Ability to meet consistent attendance.
- Ability to interact with colleagues, supervisors, and customers face to face.
General research laboratory environment. Will work with bloodborne pathogens, hazardous chemicals, and radioactive materials. Will be required to attend safety training for Bloodborne Pathogens, Laboratory Safety, and Radiation Safety. Will work extensively with mice and will be required to undergo relevant training. Employee will be required to wear appropriate protective equipment such as gloves, lab coat, and eyewear. Working conditions will require working at the bench in a cell and molecular biology laboratory, working with mice and other animal models in the laboratory and in the animal facility,
Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals" appropriate Federal Animal Welfare Regulations, and the Public Health Service "Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.