Summer Intern - Immunology: Human Antigen-Specific T Cell AIM Assay Development

Summer Intern Immunology Human AntigenSpecific T Cell AIM Assay Development - Careers at Allen Institute div div div div p /p div /div div /div /div /div div/div /div div/div /div div div/div div div div /div div div pstrongJob Openings at the Allen Institute/strong/p pAre you ready for new challenges and new opportunities? Join our team in Seattle! br /Current job opportunities are posted here as they become available./p /div div a/a a href="job-openings.php?search=true" Back To Openings /adiv/div/div /div /div /div div divp /p div /div div /div div /div div /div/div h2Summer Intern � Immunology: Human Antigen-Specific T Cell AIM Assay Development/h2 bDepartment:/b IM - Flow Cytometry divdiva href="https://apply.hrmdirect.com/resumedirect/ApplyOnline/Apply.aspx?req_id=3618597source=3618597-CJB-0"Start Your Application/a/divdiv/div/div divpstrongemSummer Intern � Immunology: Human Antigen-Specific T Cell AIM Assay Development/em/strong/p pThe mission of the Allenstrong /strongInstitute is to understand the principles that govern life and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world./p pThe mission of the Allen Institute for Immunology is to advance the fundamental understanding of human immunology through the study of immune health and diseases where excessive or impaired immune responses drive pathological processes. The Institute will employ a multi-disciplinary team approach in collaboration with academic centers of human immunology to stronggenerate novel mechanistic insights/strong into the immune synapse in health and in diseases such as autoimmunity or oncology. The Institute will simultaneously provide a strongfoundational data set/strong and tools for future immunological research as well as a novel collaboration portal for the broader scientific community./p pWe are seeking a summer intern to join the Flow Cytometry department for the summer of 2026. They will contribute to the development of a human antigen-specific T cell Activation-Induced Marker (AIM) assay, bridging flow cytometry and cellular immunology techniques. This project focuses on detecting antigen-specific (Flu/CMV) T cell activation using HLA-typed human PBMC samples and specific peptides. Over the 10-week internship, the intern will learn and apply immune cell culture techniques, gain an introduction to flow cytometry and multiplexed proteomic assays and optimize antigen-specific activation conditions to aid in implementation of this assay. The intern will review literature and existing protocols and work with mentors to define time points for cell resting and cell stimulation steps in the assay. Responsibilities include hands-on setup of PBMC cultures, incubation with Flu, CMV and control peptides, antibody staining and fixation for flow cytometry, and MSD multiplex cytokine workflows. This project provides the intern with robust experiences in single-cell immunology techniques and assay design, with outcomes suitable for inclusion in a T cell AIM assay presentation and poster session. This work will help optimize crucial steps in existing T cell AIM assays under development. This assay model, if successfully developed, could be applied to several ongoing autoimmune and oncology projects./p pAt the Allen Institute, we believe that science is for everyone � and should be open to everyone. We are dedicated to combating biases and reducing barriers to STEM careers more broadly. /p pWe also believe that science is better when it includes different perspectives and voices. We strive to make the Allen Institute a place where everyone feels like they belong and are empowered to do their best work in a supportive environment. /p pWe are an equal-opportunity employer and strongly encourage people from all backgrounds to apply for our open positions. /p pstrongemPlease include a brief personal statement with your application. See Applicant Requirements section below for details./em/strong/p pstrongEducational Objectives/strong/p ul liLearn how to safely handle and culture human blood cells (PBMC) in a BSL2 laboratory; complete Allen Institute Biosafety and general laboratory practice training module/li liCellular immunology techniques: Thaw, count, culture, and activate primary human PBMCs/li liFlow cytometry: Learn how to operate a Beckman CytoFlex LX spectral flow cytometer, analyze and process data through FlowJo or CellEngine software/li liMSD multiplex cytokine assays: Learn how to set up and analyze quantitative multiplex cytokine assays using MSD, ECL technology platform/li liAssay design and protocol optimization: Adjust conditions for cell resting and stimulation to ensure robust, reproducible results/li liScientific research methods: Engage with literature and established methods to inform project design and execution /li /ul pstrongRequired Knowledge and Experience/strong/p ul liInterest in, and basic understanding of immunology/li liBasic Microsoft computer skills including Word, Excel, and PowerPoint/li /ul pstrongApplicant Requirements/strong/p ul liMust be enrolled in, or a 2026 graduate of an accredited associate�s, bachelor�s, or master�s degree program/li liMust be able to start on June 1st, June 15th, or June 29th, 2026 and commit to the full 10-week program/li liMust be eligible to work in the United States for the duration of the internship/li liMust be 18 years of age or older/li listrongPlease upload a 300-500 word personal statement addressing the following topics:/strong ul liWhy you are interested in working at the Allen Institute/li liWhy you are interested in a summer internship and how this opportunity will be meaningful to you and your career/li liWhy you are interested in this specific role and project/li /ul /li /ul pstrongWork Environment /strong/p ul liLaboratory environment - possible exposure to chemical, biological, or other hazardous substances/li liPossible exposure to BioSafety Level 2 organisms, such as recombinant viruses/li liThis may include wearing personnel protective equipment (PPE)/li liWorking with dry ice or liquid nitrogen/li liSample handling of the following material, including but not limited to: human whole blood, PBMC, Plasma, nucleic acids, and cells/li /ul pstrongPhysical Demands/strong/p ul liOccasional lifting up to 30 pounds/li liFine motor movements in fingers/hands to operate equipment/li liRepetitive motion with lab equipment/li /ul pstrongPosition Type/Expected Hours of Work/strong/p ul liInterns are expected to work 35-40 hours a week throughout the 10-week period/li liThis role is currently working onsite and is expected to work onsite for the majority of working hours. The primary work location for this role is 615 Westlake Ave N., with flexibility to work remotely on a limited basis. /li /ul pstrongAdditional Comments/strong/p ul li**Please note, this opportunity requires U.S work authorization and does not sponsor work visas**/li li**Travel and/or housing stipend available to qualifying interns**/li /ul pstrongCompensation/strong/p ul li$22.50 per hour (non-negotiable)/li /ul pstrongBenefits/strong/p ul liInterns will accrue paid sick days and receive a free Seattle transportation card (ORCA Business Passport)/li /ul pemIt is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities./em/p /div divdiva href="https://apply.hrmdirect.com/resumedirect/ApplyOnline/Apply.aspx?req_id=3618597source=3618597-CJB-0"Start Your Application/a/divdiv/div/div /div divp /p div /div div /div div /div div /div/div divp /p div /div div /div/divdiv br /a href="//www.alleninstitute.org"Visit Our Home Page/a /divp© 2026 Allen Institute/p p a href="https://www.clearcompany.com" iApplicant Tracking System Powered by/i  /a /p /div