Biologist

Job Title: Biologist
Job Location: Boston, MA
Job Duration: 6 Months
Work shift: 1st Shift
Pay Range: $74.83 - $88.04/Hour

Job Description:
Key position requirements/responsibilities include:
Technical/scientific skills. Expertise in the following is a must-have:

• Aseptic cell culture techniques with mammalian cells.
• Transient transfections and generation of stable cell lines.
• Development of cell-based functional assays, biochemical and enzymatic assays and screening of Client therapeutics in 96-/384-well plate formats at a single concentration or in a concentration/response manner.
• Knowledge of basic pharmacology (e.g. concentration/response curves, determination of binding affinity, potency, Hill’s slope and in vitro efficacy). Mechanism of action studies of drugs.
• Experience with colorimetric, luminescence and fluorescence-based readouts, including HTRF. Proficient in operating plate readers, liquid handling robots and assay automation.
• Design, develop, and optimize in vitro enzymatic assays e.g., ADP-Glo, Kinase-Glo assays
• Gene expression studies by qPCR, western blot and FACS techniques.
• Soft skills:
• Proficient oral and written communication skills and ability to work both independently and in teams.
• Requirement for accurate record keeping and independent data analysis. Will perform work in full compliance with Client Research Policies and complete all required training activities in the timeframe specified.
• Attention to detail and ability to prioritize work on multiple projects, timelines and company goals.
• Eager to grasp fundamental principles, swiftly acquire new abilities, and adjust to Client challenges.

Minimum qualifications:

• B.S. and/or M.S. in biology, pharmacology, biochemistry or related field.
• 8-10 years of laboratory experience.

Additional Skills/Preferences:

• Experience/expertise with the following is a plus:
• Native cell lines and primary cell cultures, iPSCs, co-cultures, 3D cultures.
• Industry experience.
• Exposure to multiple therapeutic Client classes (e.g. membrane receptors, including G-Protein Coupled Receptors or GPCRs, enzymes, transporters, etc.) and multiple modalities (small molecules, pepProducts, antibodies, siRNAs, etc.).
• Experience with ELISA assays.
• Working knowledge or background in cardiometabolic diseases.

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