Scientist

Job Title: Scientist
Duration: 12months
Location/Site: Worcester, MA - 100% Onsite; 01604

Description:
This opportunity is in the Cell Line Engineering group within Biotherapeutics Discovery which plays a key role in enabling reagent generation supporting diverse applications throughout Client's therapeutic development process. Under supervision of a senior scientist in the group, the individual will primarily support mammalian cell culture work, cell line engineering, including KO, KI and OE cell lines development, cellular assays, as well as assist with lab and inventory management and sample digitization initiatives. Opportunities to expand proficiencies in cell and molecular biology applications, such as cell and genome engineering, vector design, technology development, cell-based assays development, receptor density quantification, flow cytometry, cell sorting and cloning, and other cell line development technologies also exist.

What are the top 3-5 skills, experience or education required for this position:
1. MSc (non PhD) with 2-3 years of industry experience
2. Mammalian cell culture and cell line development
3. Flow cytometry (analysis and sorting)
4. Gene editing tools (CRISPR, ZFN, TALENS, recombinases)
5. Cell-based assays

Key responsibilities include:

• Mammalian cell culture (immortalized, cancer and primary lines)
• Generation and maintenance of cell-lines for antibody discovery and assay development.
• Cellular assays
• FACS analysis and sorting
• Technology development for cell line engineering
• Consolidation of historical and recent cell line assets into digital inventory solution.
• Expansion and banking of core cell lines to ensure adequate inventories.
• Coordination of cell line inventory stocks across multiple Client's sites, providing a foundation for Cell Bank establishment.